RESUMO
A convenient method of regioselective introduction of 1-beta-D-galactopyranosylthymine into oligonucleotides was developed and the substrate properties of the modified oligonucleotides were investigated in the enzymic reaction of formation and hydrolysis of internucleotide bonds. The English version of the paper.
Assuntos
Bioquímica/métodos , Galactose/química , Galactose/metabolismo , Oligonucleotídeos/química , Oligonucleotídeos/metabolismo , Timina/química , Timina/metabolismo , Desoxirribonuclease EcoRI/química , Desoxirribonuclease EcoRI/metabolismo , Desoxirribonucleases de Sítio Específico do Tipo II/química , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Galactose/análogos & derivados , Hidrólise , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Relação Estrutura-Atividade , Timina/análogos & derivadosRESUMO
The employment of periodate oxidation in the chemistry of nucleic acids and their components is reviewed. The reaction mechanism, structural requirements to substrates, and synthesis of dialdehyde derivatives of nucleosides, nucleotides, and oligonucleotides are discussed in the first part. The second part involves chemical, physicochemical, and biological properties of the dialdehyde derivatives, as well as their use for the affinity modifications of proteins.
